prolong gold antifade mountant with dna stain dapi Search Results


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A. HGPS (HGADFN127) fibroblasts were labeled with <t>DAPI</t> <t>(DNA,</t> blue), and anti-progerin antibody (red). Representative images exhibiting chromatin lagging during the different stages of mitosis are shown. Arrowheads indicate the position of chromatin lagging. B. Binucleated cells were labeled with DAPI (blue) and calnexin (green); a representative image is shown. Double merged signals for progerin with DNA or calnexin with DNA are shown. Scale bar, 10 μm. C. The numbers of cells exhibiting lagging chromatin at different stages of mitosis were scored as described in the Materials and Methods in control (GMO3349C, GMO1651C) and HGPS cells (HGADFN127, HGADFN003). The average mitotic index was on average 1.40% in control cultures and 0.94% in HGPS cultures. Quantification of mitotic cells exhibiting lagging chromatin was scored for each stage of mitosis and the percentage relative to the total number of mitotic cells present in control or HGPS cultures was determined as indicated. A total number of 5217 cells were screened in control and 4976 in HGPS cultures out of 6 independent cultures for each control and HGPS cell lines. The values are reported as means ± SD and evaluated using Student's t-test. D. The percentage of binucleated cells and cells with giant nuclei were scored by direct count of a total of 1559 control (GMO3349C, GMO1651C) cells and 2130 HGPS (HGADFN127, HGADFN003) cells derived from 3 independent cultures. Data are presented as means ± SD and evaluated using Student's t-test. E. In parallel to the evaluation described in (C), the numbers of cells found at a specific mitotic stage were scored and determined as described in (C). Mitotic HGPS cells were found to be increased during anaphase.
Dapi, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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A. HGPS (HGADFN127) fibroblasts were labeled with <t>DAPI</t> <t>(DNA,</t> blue), and anti-progerin antibody (red). Representative images exhibiting chromatin lagging during the different stages of mitosis are shown. Arrowheads indicate the position of chromatin lagging. B. Binucleated cells were labeled with DAPI (blue) and calnexin (green); a representative image is shown. Double merged signals for progerin with DNA or calnexin with DNA are shown. Scale bar, 10 μm. C. The numbers of cells exhibiting lagging chromatin at different stages of mitosis were scored as described in the Materials and Methods in control (GMO3349C, GMO1651C) and HGPS cells (HGADFN127, HGADFN003). The average mitotic index was on average 1.40% in control cultures and 0.94% in HGPS cultures. Quantification of mitotic cells exhibiting lagging chromatin was scored for each stage of mitosis and the percentage relative to the total number of mitotic cells present in control or HGPS cultures was determined as indicated. A total number of 5217 cells were screened in control and 4976 in HGPS cultures out of 6 independent cultures for each control and HGPS cell lines. The values are reported as means ± SD and evaluated using Student's t-test. D. The percentage of binucleated cells and cells with giant nuclei were scored by direct count of a total of 1559 control (GMO3349C, GMO1651C) cells and 2130 HGPS (HGADFN127, HGADFN003) cells derived from 3 independent cultures. Data are presented as means ± SD and evaluated using Student's t-test. E. In parallel to the evaluation described in (C), the numbers of cells found at a specific mitotic stage were scored and determined as described in (C). Mitotic HGPS cells were found to be increased during anaphase.
Recombinant Proteins Vectashield Plus Dapi Vector Sku H 1200 10 Vectashield Vector Sku H 1000 10 Dapi Sigma Aldrich, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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A. HGPS (HGADFN127) fibroblasts were labeled with <t>DAPI</t> <t>(DNA,</t> blue), and anti-progerin antibody (red). Representative images exhibiting chromatin lagging during the different stages of mitosis are shown. Arrowheads indicate the position of chromatin lagging. B. Binucleated cells were labeled with DAPI (blue) and calnexin (green); a representative image is shown. Double merged signals for progerin with DNA or calnexin with DNA are shown. Scale bar, 10 μm. C. The numbers of cells exhibiting lagging chromatin at different stages of mitosis were scored as described in the Materials and Methods in control (GMO3349C, GMO1651C) and HGPS cells (HGADFN127, HGADFN003). The average mitotic index was on average 1.40% in control cultures and 0.94% in HGPS cultures. Quantification of mitotic cells exhibiting lagging chromatin was scored for each stage of mitosis and the percentage relative to the total number of mitotic cells present in control or HGPS cultures was determined as indicated. A total number of 5217 cells were screened in control and 4976 in HGPS cultures out of 6 independent cultures for each control and HGPS cell lines. The values are reported as means ± SD and evaluated using Student's t-test. D. The percentage of binucleated cells and cells with giant nuclei were scored by direct count of a total of 1559 control (GMO3349C, GMO1651C) cells and 2130 HGPS (HGADFN127, HGADFN003) cells derived from 3 independent cultures. Data are presented as means ± SD and evaluated using Student's t-test. E. In parallel to the evaluation described in (C), the numbers of cells found at a specific mitotic stage were scored and determined as described in (C). Mitotic HGPS cells were found to be increased during anaphase.
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Beijing Solarbio Science dapi
A. HGPS (HGADFN127) fibroblasts were labeled with <t>DAPI</t> <t>(DNA,</t> blue), and anti-progerin antibody (red). Representative images exhibiting chromatin lagging during the different stages of mitosis are shown. Arrowheads indicate the position of chromatin lagging. B. Binucleated cells were labeled with DAPI (blue) and calnexin (green); a representative image is shown. Double merged signals for progerin with DNA or calnexin with DNA are shown. Scale bar, 10 μm. C. The numbers of cells exhibiting lagging chromatin at different stages of mitosis were scored as described in the Materials and Methods in control (GMO3349C, GMO1651C) and HGPS cells (HGADFN127, HGADFN003). The average mitotic index was on average 1.40% in control cultures and 0.94% in HGPS cultures. Quantification of mitotic cells exhibiting lagging chromatin was scored for each stage of mitosis and the percentage relative to the total number of mitotic cells present in control or HGPS cultures was determined as indicated. A total number of 5217 cells were screened in control and 4976 in HGPS cultures out of 6 independent cultures for each control and HGPS cell lines. The values are reported as means ± SD and evaluated using Student's t-test. D. The percentage of binucleated cells and cells with giant nuclei were scored by direct count of a total of 1559 control (GMO3349C, GMO1651C) cells and 2130 HGPS (HGADFN127, HGADFN003) cells derived from 3 independent cultures. Data are presented as means ± SD and evaluated using Student's t-test. E. In parallel to the evaluation described in (C), the numbers of cells found at a specific mitotic stage were scored and determined as described in (C). Mitotic HGPS cells were found to be increased during anaphase.
Dapi, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cytoskeleton Inc nuclear counterstain dapi
A. HGPS (HGADFN127) fibroblasts were labeled with <t>DAPI</t> <t>(DNA,</t> blue), and anti-progerin antibody (red). Representative images exhibiting chromatin lagging during the different stages of mitosis are shown. Arrowheads indicate the position of chromatin lagging. B. Binucleated cells were labeled with DAPI (blue) and calnexin (green); a representative image is shown. Double merged signals for progerin with DNA or calnexin with DNA are shown. Scale bar, 10 μm. C. The numbers of cells exhibiting lagging chromatin at different stages of mitosis were scored as described in the Materials and Methods in control (GMO3349C, GMO1651C) and HGPS cells (HGADFN127, HGADFN003). The average mitotic index was on average 1.40% in control cultures and 0.94% in HGPS cultures. Quantification of mitotic cells exhibiting lagging chromatin was scored for each stage of mitosis and the percentage relative to the total number of mitotic cells present in control or HGPS cultures was determined as indicated. A total number of 5217 cells were screened in control and 4976 in HGPS cultures out of 6 independent cultures for each control and HGPS cell lines. The values are reported as means ± SD and evaluated using Student's t-test. D. The percentage of binucleated cells and cells with giant nuclei were scored by direct count of a total of 1559 control (GMO3349C, GMO1651C) cells and 2130 HGPS (HGADFN127, HGADFN003) cells derived from 3 independent cultures. Data are presented as means ± SD and evaluated using Student's t-test. E. In parallel to the evaluation described in (C), the numbers of cells found at a specific mitotic stage were scored and determined as described in (C). Mitotic HGPS cells were found to be increased during anaphase.
Nuclear Counterstain Dapi, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Nikon a1r confocal microscope
A. HGPS (HGADFN127) fibroblasts were labeled with <t>DAPI</t> <t>(DNA,</t> blue), and anti-progerin antibody (red). Representative images exhibiting chromatin lagging during the different stages of mitosis are shown. Arrowheads indicate the position of chromatin lagging. B. Binucleated cells were labeled with DAPI (blue) and calnexin (green); a representative image is shown. Double merged signals for progerin with DNA or calnexin with DNA are shown. Scale bar, 10 μm. C. The numbers of cells exhibiting lagging chromatin at different stages of mitosis were scored as described in the Materials and Methods in control (GMO3349C, GMO1651C) and HGPS cells (HGADFN127, HGADFN003). The average mitotic index was on average 1.40% in control cultures and 0.94% in HGPS cultures. Quantification of mitotic cells exhibiting lagging chromatin was scored for each stage of mitosis and the percentage relative to the total number of mitotic cells present in control or HGPS cultures was determined as indicated. A total number of 5217 cells were screened in control and 4976 in HGPS cultures out of 6 independent cultures for each control and HGPS cell lines. The values are reported as means ± SD and evaluated using Student's t-test. D. The percentage of binucleated cells and cells with giant nuclei were scored by direct count of a total of 1559 control (GMO3349C, GMO1651C) cells and 2130 HGPS (HGADFN127, HGADFN003) cells derived from 3 independent cultures. Data are presented as means ± SD and evaluated using Student's t-test. E. In parallel to the evaluation described in (C), the numbers of cells found at a specific mitotic stage were scored and determined as described in (C). Mitotic HGPS cells were found to be increased during anaphase.
A1r Confocal Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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A. HGPS (HGADFN127) fibroblasts were labeled with <t>DAPI</t> <t>(DNA,</t> blue), and anti-progerin antibody (red). Representative images exhibiting chromatin lagging during the different stages of mitosis are shown. Arrowheads indicate the position of chromatin lagging. B. Binucleated cells were labeled with DAPI (blue) and calnexin (green); a representative image is shown. Double merged signals for progerin with DNA or calnexin with DNA are shown. Scale bar, 10 μm. C. The numbers of cells exhibiting lagging chromatin at different stages of mitosis were scored as described in the Materials and Methods in control (GMO3349C, GMO1651C) and HGPS cells (HGADFN127, HGADFN003). The average mitotic index was on average 1.40% in control cultures and 0.94% in HGPS cultures. Quantification of mitotic cells exhibiting lagging chromatin was scored for each stage of mitosis and the percentage relative to the total number of mitotic cells present in control or HGPS cultures was determined as indicated. A total number of 5217 cells were screened in control and 4976 in HGPS cultures out of 6 independent cultures for each control and HGPS cell lines. The values are reported as means ± SD and evaluated using Student's t-test. D. The percentage of binucleated cells and cells with giant nuclei were scored by direct count of a total of 1559 control (GMO3349C, GMO1651C) cells and 2130 HGPS (HGADFN127, HGADFN003) cells derived from 3 independent cultures. Data are presented as means ± SD and evaluated using Student's t-test. E. In parallel to the evaluation described in (C), the numbers of cells found at a specific mitotic stage were scored and determined as described in (C). Mitotic HGPS cells were found to be increased during anaphase.
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Santa Cruz Biotechnology dna
A. HGPS (HGADFN127) fibroblasts were labeled with <t>DAPI</t> <t>(DNA,</t> blue), and anti-progerin antibody (red). Representative images exhibiting chromatin lagging during the different stages of mitosis are shown. Arrowheads indicate the position of chromatin lagging. B. Binucleated cells were labeled with DAPI (blue) and calnexin (green); a representative image is shown. Double merged signals for progerin with DNA or calnexin with DNA are shown. Scale bar, 10 μm. C. The numbers of cells exhibiting lagging chromatin at different stages of mitosis were scored as described in the Materials and Methods in control (GMO3349C, GMO1651C) and HGPS cells (HGADFN127, HGADFN003). The average mitotic index was on average 1.40% in control cultures and 0.94% in HGPS cultures. Quantification of mitotic cells exhibiting lagging chromatin was scored for each stage of mitosis and the percentage relative to the total number of mitotic cells present in control or HGPS cultures was determined as indicated. A total number of 5217 cells were screened in control and 4976 in HGPS cultures out of 6 independent cultures for each control and HGPS cell lines. The values are reported as means ± SD and evaluated using Student's t-test. D. The percentage of binucleated cells and cells with giant nuclei were scored by direct count of a total of 1559 control (GMO3349C, GMO1651C) cells and 2130 HGPS (HGADFN127, HGADFN003) cells derived from 3 independent cultures. Data are presented as means ± SD and evaluated using Student's t-test. E. In parallel to the evaluation described in (C), the numbers of cells found at a specific mitotic stage were scored and determined as described in (C). Mitotic HGPS cells were found to be increased during anaphase.
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PeproTech dapi
A. HGPS (HGADFN127) fibroblasts were labeled with <t>DAPI</t> <t>(DNA,</t> blue), and anti-progerin antibody (red). Representative images exhibiting chromatin lagging during the different stages of mitosis are shown. Arrowheads indicate the position of chromatin lagging. B. Binucleated cells were labeled with DAPI (blue) and calnexin (green); a representative image is shown. Double merged signals for progerin with DNA or calnexin with DNA are shown. Scale bar, 10 μm. C. The numbers of cells exhibiting lagging chromatin at different stages of mitosis were scored as described in the Materials and Methods in control (GMO3349C, GMO1651C) and HGPS cells (HGADFN127, HGADFN003). The average mitotic index was on average 1.40% in control cultures and 0.94% in HGPS cultures. Quantification of mitotic cells exhibiting lagging chromatin was scored for each stage of mitosis and the percentage relative to the total number of mitotic cells present in control or HGPS cultures was determined as indicated. A total number of 5217 cells were screened in control and 4976 in HGPS cultures out of 6 independent cultures for each control and HGPS cell lines. The values are reported as means ± SD and evaluated using Student's t-test. D. The percentage of binucleated cells and cells with giant nuclei were scored by direct count of a total of 1559 control (GMO3349C, GMO1651C) cells and 2130 HGPS (HGADFN127, HGADFN003) cells derived from 3 independent cultures. Data are presented as means ± SD and evaluated using Student's t-test. E. In parallel to the evaluation described in (C), the numbers of cells found at a specific mitotic stage were scored and determined as described in (C). Mitotic HGPS cells were found to be increased during anaphase.
Dapi, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


A. HGPS (HGADFN127) fibroblasts were labeled with DAPI (DNA, blue), and anti-progerin antibody (red). Representative images exhibiting chromatin lagging during the different stages of mitosis are shown. Arrowheads indicate the position of chromatin lagging. B. Binucleated cells were labeled with DAPI (blue) and calnexin (green); a representative image is shown. Double merged signals for progerin with DNA or calnexin with DNA are shown. Scale bar, 10 μm. C. The numbers of cells exhibiting lagging chromatin at different stages of mitosis were scored as described in the Materials and Methods in control (GMO3349C, GMO1651C) and HGPS cells (HGADFN127, HGADFN003). The average mitotic index was on average 1.40% in control cultures and 0.94% in HGPS cultures. Quantification of mitotic cells exhibiting lagging chromatin was scored for each stage of mitosis and the percentage relative to the total number of mitotic cells present in control or HGPS cultures was determined as indicated. A total number of 5217 cells were screened in control and 4976 in HGPS cultures out of 6 independent cultures for each control and HGPS cell lines. The values are reported as means ± SD and evaluated using Student's t-test. D. The percentage of binucleated cells and cells with giant nuclei were scored by direct count of a total of 1559 control (GMO3349C, GMO1651C) cells and 2130 HGPS (HGADFN127, HGADFN003) cells derived from 3 independent cultures. Data are presented as means ± SD and evaluated using Student's t-test. E. In parallel to the evaluation described in (C), the numbers of cells found at a specific mitotic stage were scored and determined as described in (C). Mitotic HGPS cells were found to be increased during anaphase.

Journal: Oncotarget

Article Title: Progerin impairs chromosome maintenance by depleting CENP-F from metaphase kinetochores in Hutchinson-Gilford progeria fibroblasts

doi: 10.18632/oncotarget.8267

Figure Lengend Snippet: A. HGPS (HGADFN127) fibroblasts were labeled with DAPI (DNA, blue), and anti-progerin antibody (red). Representative images exhibiting chromatin lagging during the different stages of mitosis are shown. Arrowheads indicate the position of chromatin lagging. B. Binucleated cells were labeled with DAPI (blue) and calnexin (green); a representative image is shown. Double merged signals for progerin with DNA or calnexin with DNA are shown. Scale bar, 10 μm. C. The numbers of cells exhibiting lagging chromatin at different stages of mitosis were scored as described in the Materials and Methods in control (GMO3349C, GMO1651C) and HGPS cells (HGADFN127, HGADFN003). The average mitotic index was on average 1.40% in control cultures and 0.94% in HGPS cultures. Quantification of mitotic cells exhibiting lagging chromatin was scored for each stage of mitosis and the percentage relative to the total number of mitotic cells present in control or HGPS cultures was determined as indicated. A total number of 5217 cells were screened in control and 4976 in HGPS cultures out of 6 independent cultures for each control and HGPS cell lines. The values are reported as means ± SD and evaluated using Student's t-test. D. The percentage of binucleated cells and cells with giant nuclei were scored by direct count of a total of 1559 control (GMO3349C, GMO1651C) cells and 2130 HGPS (HGADFN127, HGADFN003) cells derived from 3 independent cultures. Data are presented as means ± SD and evaluated using Student's t-test. E. In parallel to the evaluation described in (C), the numbers of cells found at a specific mitotic stage were scored and determined as described in (C). Mitotic HGPS cells were found to be increased during anaphase.

Article Snippet: DNA was stained with DAPI (Vector Inc., VEC-H-1200).

Techniques: Labeling, Derivative Assay